The Qubit 4 Flurometer is the latest version of the popular Qubit fluorometer designed to accurately measure DNA, RNA, and protein quantity, and now also RNA integrity and quality, using the highly sensitive Qubit assays. The Qubit 4 Fluorometer was re-engineered to run the Qubit RNA IQ (integrity & quality) assay. The Qubit 4 Fluorometer and RNA IQ Assay Kit work together to accurately distinguish intact from degraded RNA in just two easy steps.

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For all Qubit assays, the concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result. The easy-to-use touch-screen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.

Key features of the Qubit 4 Fluorometer include:
• Fast and highly accurate quantitation of DNA, RNA, and protein in less than three seconds per sample
• Measures intact RNA in less than 5 seconds per sample
• High levels of accuracy using only 1–20 μL of sample, even with very dilute samples
• On-board Reagent Calculator quickly generates Qubit working solution preparation instructions
• Stores results from up to 1000 samples
• Large 5.7-inch, state-of-the-art color touch screen for easy workflow navigation
• Graphical display indicates when samples are in the extended range or out of range
• Small footprint saves space on your bench
• Exports data to a USB drive or directly to your computer via a USB cable
• Ability to personalize your Qubit fluorometer with the assays you run most, add new assays, or even create your own assays with the MyQubit software and web tool
• Language of your choice including English, French, Spanish, Italian, German, simplified Chinese, and Japanese

The Qubit 4 Flurometer can also be used to directly measure the fluorescence of samples using Fluorometer mode. Additionally, Ion Sphere Particle quality can be evaluated on the Qubit 4 Fluorometer using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion PGM sequencer.

Enables more accurate results than with UV absorbance at low concentrations
The Qubit 4 Fluorometer is far more sensitive than UV absorbance, which measures anything absorbing at 260 nm, including DNA, RNA, protein, free nucleotides, or excess salts. Moreover, UV spectrophotometry is often not sensitive enough to accurately measure low concentrations of DNA and RNA. With the Qubit fluorometer, your research is enhanced by more accurate measurements because the dyes in the Qubit assay kits fluoresce only when bound to the selected molecule (DNA, RNA or protein) in your sample. This allows you to avoid repeating work due to inaccurate measurements.

Fast, simple, and accurate measurement of RNA quality
The Qubit RNA IQ assay provides a fast, simple method to check whether an RNA sample has degraded. The assay utilizes two unique dyes: one that binds to large, intact RNA, and another that selectively binds to small, degraded RNA. Together they enable you to quickly assess the quality and integrity of an RNA sample. To use, simply add your samples to the RNA IQ working solution, then measure on the Qubit 4 Fluorometer.

Save samples and bench space
The thoughtfully designed Qubit 4 Fluorometer requires samples of only 1–20 µL. It is intended for use at room temperature and occupies only a small amount of bench space. The Qubit 4 Fluorometer features advanced optics and data analysis algorithms, and comes equipped with a USB thumb drive and cable for data transfer to Excel™ software and for software downloads, as well as a universal power supply, four plug adapters, and electromagnetic CE certification.

Single sample measurements are a breeze
This user-friendly fluorometer is combined with quick and simple assay procedures. Calculations and settings are automatically performed by the instrument. The Qubit assays for use with the Qubit 4 Fluorometer are all performed using the same general protocol, which uses a simple mix-and-read format with incubation times of only two minutes for the DNA and RNA assays. 500 mL thin-walled PCR tubes (Cat. No. Q32856) are required.

System specifications include:

Instrument dimensions: 5.4"(w) x 10" (l) x 2.2" (h) (13.6 cm x 25 cm x 5.5 cm)
Weight: 743 g
Dynamic range: 5 orders of magnitude
Processing time: ≤seconds/sample
Light sources: blue LED (max ~470 nm), red LED (max ~635 nm)
Excitation filters: blue (430–495 nm), red (600–645 nm)
Emission filters: green (510–580 nm), red (665–720 nm)
Detectors: photodiodes, measurement capability from 300–1,000 nm
Warm-up time: <35 seconds
USB thumb drive: 4 GB

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Cat No Description Size Price Promo Price
Q33227 Qubit™ 4 Quantitation Starter Kit 1 Kit €3,017.00 €2,450.00
  The Qubit 4 Quantitation Starter Kit includes:      
  • One Qubit 4 Fluorometer (Cat. No. Q33226)      
  • Qubit Assay Tubes (set of 500) (Cat. No. Q32856)      
  • Qubit 1X dsDNA HS Assay Kit, 100 assays (Cat. No. Q33230)      
  • Qubit dsDNA BR Assay Kit, 100 assays (Cat. No. Q32850)      
  • Qubit RNA IQ Assay Kit, 75 assays (Cat. No. Q33221)      
  • Qubit RNA HS Assay Kit, 100 assays (Cat. No. Q32852)      
  • Qubit RNA XR Assay Kit, 100 assays (Cat. No. Q33223)      
  • Qubit Protein Assay Kit, 100 assays (Cat. No. Q33211)      
         
Cat No Description Size Price Discounted Price
Q33228 Qubit™ 4 NGS Starter Kit 1 Kit €2,946.00 €2,533.56
  The Qubit 4 NGS Starter Kit for next-generation sequencing includes:      
  • One Qubit 4 Fluorometer (Cat. No. Q33226)      
  • Qubit Assay Tubes (set of 500) (Cat. No. Q32856)      
  • Qubit 1X dsDNA HS Assay Kit, 500 assays (Cat. No. Q33231)      
         
Q33229 Qubit™ 4 RNA IQ Starter Kit 1 Kit €3,092.00 €2,659.12
  The Qubit 4 RNA IQ Starter Kit includes:      
  • One Qubit 4 Fluorometer (Cat. No. Q33226)      
  • Qubit Assay Tubes (set of 500) (Cat. No. Q32856)      
  • Qubit RNA IQ Assay Kit, 275 assays (Cat. No. 33222)      
         
Q33226 Qubit™ 4 Fluorometer   €2,786.00 €2,395.96
         
Cat No Description Size Price Discounted Price
  Kits and Accessories      
Q32850 Qubit™ dsDNA BR Assay Kit 100 assays €108.16 €74.92
Q32853 Qubit™ dsDNA BR Assay Kit 500 assays €308.75 €255.00
Q33230 Qubit™ 1X dsDNA HS Assay Kit 100 assays €100.23 €86.20
Q33231 Qubit™ 1X dsDNA HS Assay Kit 500 assays €302.32 €260.00
Q33232 Quant-iT™ 1X dsDNA HS Assay Kit 1000 assays €437.00 €376.00
Q33233 Qubit™ 1X dsDNA HS Assay Lambda Standard 5 mL €60.93 €52.40
Q10212 Qubit™ ssDNA Assay Kit 1 kit €112.52 €96.75
Q33221 Qubit™ RNA IQ Assay Kit 75 assays €153.48 €132.00
Q33222 Qubit™ RNA IQ Assay Kit 275 assays €463.95 €399.00
Q33223 Qubit™ RNA XR Assay Kit 100 assays €95.00 €81.70
Q33224 Qubit™ RNA XR Assay Kit 500 assays €286.00 €246.00
Q33235 Qubit™ RNA IQ Standards 1 set €60.93 €52.40
Q33236 Qubit™ RNA XR Standard 5 each €60.93 €52.40
Q32852 Qubit™ RNA HS Assay Kit 100 assays €84.90 €73.00
Q32855 Qubit™ RNA HS Assay Kit 500 assays €296.57 €255.00
Q33223 Qubit™ RNA XR Assay Kit 100 assays €95.00 €81.70
Q33224 Qubit™ RNA XR Assay Kit 500 assays €286.00 €246.00
Q33225 Quant-iT™ RNA XR Assay Kit 1000 assays €396.61 €341.00
Q32880 Qubit™ microRNA Assay Kit 100 assays €110.19 €94.75
Q32881 Qubit™ microRNA Assay Kit 500 assays €330.29 €284.00
Q33211 Qubit™ Protein Assay Kit 100 assays €113.39 €97.50
Q33212 Qubit™ Protein Assay Kit 500 assays €336.11 €289.00
Q32856 Qubit™ Assay Tubes 500 tubes €75.26 €64.71
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